Ethanol 95% SDA (190 Proof) - 1000 mL;

Ethanol 95, recommended laboratory reagent and solvent.  Component of Volu-Sol's Parasitology Kit INTENDED USE: For use in the rapid staining and differentiation of intestinal parasites. According to the Gomori procedure it can be used to counterstain histologic sections. SUMMARY AND PRINCIPLES: Claude Masson introduced the first trichrome stain in 1929. It offered a simple, quick alternative to the tissue stains previously used. In 1949, George Gomori developed a single solution trichrome stain as a counterstain for hematoxylin stained tissue sections and cytological smears. Peter Wheatley's observation of E. Histolytica stained with chromotrope 2R in 1951 yielded the Wheatley methodology. The modification of traditional fixation and dehydration procedures, combined with trichrome stain, resulted in a procedure which stains amoeba and flagellates rapidly and easily. Volu-Sol's Trichrome Stain is recommended for use according to the Wheatley procedure for parasites, as well as the Gomori procedure for tissues. Volu-Sol's PVA and Schaudinn's Modified Fixatives are copper based and do not contain mercuric chloride. This modification eliminates the iodine-alcohol step typically used in the trichrome stain Kit.